scvelo

SUMMARY

What it does

This Agent Skill provides guidance for performing RNA velocity analysis using the scVelo Python package. It includes instructions for preprocessing single-cell RNA-seq data with spliced and unspliced layers, estimating RNA velocity using stochastic or dynamical models, computing latent time and velocity pseudotime, identifying driver genes, and visualizing results on UMAP embeddings. The skill is designed to complement Scanpy workflows and requires Python 3.10+ with scvelo, scanpy, and anndata installed. The source document includes code examples and best practices.